1. Wientraub K. 2013, The new gold standard, Nature 495, S14-S16. PDF
In a recent research, Peer and colleagues, including Rotello, found that modifying the gold surface with hydrophobic residues dictates the immune system's response. In both in vitro and mouse studies, they found that an increase in hydrophobicity is correlated linearly with a rise in immune activation. Moyano D. et al (2012). Hydrophobicity Dictates Immune response. Journal of American Chemical Society 134(9), 3965-3967. PDF
2. MacLachlan I. 2008, siRNAs with guts, Nature Biotechnology 26, 403 - 405. PDF
The process described by Peer and colleagues involves formation of neutral lipid vesicles by extrusion, two independent covalent modification steps, lyophilization and, finally, rehydration of antibody-targeted liposomes with a solution containing protamine-condensed polyplex siRNA. Peer D. et al (2008). Systemic leukocyte-directed siRNA delivery revealing cyclin D1 as an anti-Inflammation target. Science 319, 627-630. PDF
3. Mullard A. 2008, Escorting siRNA, Nature Reviews Molecular Cell Biology 9, 189. PDF
4. siRNA fully loaded, 2008, Nature Chemical Biology 4, 167. PDF
5. Szoka F. 2008, The Art of Assembly, Science 319 5863, 578-579. PDF
"The work by Peer et al. is a technical tour de force. The sequential assembly process involves two chemical couplings that, if not done properly, can potentially generate Nacylurea groups on the hyaluronan. When N-acylurea groups are hydrolyzed into the ethylurea compound, they have an antiinflammatory effect. Extensive experimental controls by Peer et al. provide a high level of assurance that the anti-inflammatory effect observed results from decreased cyclin D1 expression by siRNA that is delivered to leukocytes"